ZFIN Image: van der Klaauw et al., 2019, Fig. S3

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Figure Caption

Fig. S3

Generation and Characterization of Semaphorin-Neuropilin-Plexin Deletion Mutants in Zebrafish, Related to Figure 3

(A) Schematic illustrating the mutagenesis strategy to target sema3, plxna and nrpgenes in zebrafish. Two-five sgRNAs were generated to mutagenize each zebrafish gene. Only sgRNAs verified to induce mutagenesis were injected into one-cell stage zebrafish embryos. Zebrafish were raised to ∼30 days post fertilization and fish length (mm), weight (mg) and % body fat were quantified.

(B) Results on fish length (mm), weight (mg) and % body fat for all deletion mutants (summarized in Figure 3B).

(C) Microphotographs and quantification of the density of α-melanocyte-stimulating hormone (αMSH) (red) and agouti-related peptide (AgRP) (green) immunoreactive (IR) fibers innervating the preoptic area (POA), anterior tuberal nucleus of hypothalamus (ATN), and lateral hypothalamic nucleus (LH) of 35-day-old wild-type zebrafish overexpressing NRP1 and NRP2; ^∗p < 0.05 in one-sample t tests.

Figure Data

Phenotype details

Acknowledgments

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Reprinted from Cell, 176(4), van der Klaauw, A.A., Croizier, S., Mendes de Oliveira, E., Stadler, L.K.J., Park, S., Kong, Y., Banton, M.C., Tandon, P., Hendricks, A.E., Keogh, J.M., Riley, S.E., Papadia, S., Henning, E., Bounds, R., Bochukova, E.G., Mistry, V., O'Rahilly, S., Simerly, R.B., INTERVAL, UK10K Consortium, Minchin, J.E.N., Barroso, I., Jones, E.Y., Bouret, S.G., Farooqi, I.S., Human Semaphorin 3 Variants Link Melanocortin Circuit Development and Energy Balance, 729-742.e18, Copyright (2019) with permission from Elsevier. Full text @ Cell