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Fig. 5
Full-length human SHANK3 mRNA partially rescues transit time in shank3abΔC +/− larvae. a When measuring the total percentage of fluorescent beads within the intestinal bulb (highlighted region of analysis for in magenta), total content of the long isoform (5t) rescue (dark gray) decreases at a rate quicker than the unrescued shank3abΔC +/− (black) but not as quickly as the WT (light gray). Significant differences of intestinal bulb emptying (total intestinal bulb content) were found between the long isoform rescued and unrescued shank3abΔC +/− larvae at 12 h (p < 0.006) and 24 h (p < 0.03), and between WT and unrescued shank3abΔC +/− at 6 h (p < 0.02) and 12 h (p < 0.01). bFluorescent microbead expulsion (analogous to completed digestion; highlighted region of analysis in orange) was not rescued, however, and the long isoform rescued larvae showed expulsion rates closer to shank3abΔC +/− than that of WT. Significant differences were found at 6 h between WT, the long isoform rescue, and unrescued shank3abΔC +/− (p < 0.006), at 12 h between WT and unrescued shank3abΔC +/− (p < 0.001), WT and the long isoform rescue (p < 0.001), and at 24 h between unrescued shank3abΔC +/− and the long isoform rescue larvae (p < 0.01), and WT and the long isoform rescue larvae (p < 0.005). ci Graph of bead distribution per time period for the long isoform (5t) rescue larvae (n = 19) and cii short isoform (32t) rescue larvae (n = 6). Each point represents the average +/− standard error percentage of total beads held by each region