ZFIN Image: Khalil et al., 2018, Fig. 2

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Fig. 2

Blocking the translation of dnm mRNA in zebrafish embryos causes proteinuria. Wild‐type zebrafish embryos were injected with an anti‐dnm morpholino or a scrambled control morpholino, followed by a mixture of 3 and 70 kDa dextran molecules. (A, B) Representative fluorescence images of the proximal tubule epithelial cells (circled structure) in a dynamin‐knockdown zebrafish embryo. (C, D) The number of fluorescent droplets below the luminal surface was counted in control embryos, dynamin‐knockdown embryos, and wild‐type embryos injected with puromycin aminonucleoside (PAN) as a positive control for increased glomerular permeability. *p < 0.05 versus control. A digital high‐pass filter has been placed over panels A and B to enhance the contrast between reabsorption droplets and the surrounding tissue. The images in A and B were taken at the same magnification. Scale bar = 10 μm.

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Pathol.