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Fig. 5 Rescue of the morphant phenotype by CTCF mRNA injection. Embryos were injected only with mo-aug (mo) or mo and either 50 pg or 100 pg of the human in vitro transcribed CTCF mRNA, and photographed at 24 hpf. The regions affected with the mo, and partially rescued with the CTCF mRNA are indicated: a. Forebrain, b. Midbrain, c. Hindbrain, d. Eye, e. Notochord, f. Somites. The percentage of lethal or wild type embryos, or embryos with the phenotype described in Fig. 4, was graphed.
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Reprinted from Mechanisms of Development, 154, Carmona-Aldana, F., Zampedri, C., Suaste-Olmos, F., Murillo-de-Ozores, A., Guerrero, G., Arzate-Mejía, R., Maldonado, E., Navarro, R., Chimal-Monroy, J., Recillas-Targa, F., CTCF knockout reveals an essential role for this protein during the zebrafish development, 51-59, Copyright (2018) with permission from Elsevier. Full text @ Mech. Dev.