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Fig. 3
Hnf1b is translocated to the cytoplasm in prospective CS cells.
(A) Panels show Hnf1b immunostains (graysale) on transverse cryosections of zebrafish embryos through the region of the trunk where CS glands form at 22 hpf, 36 hpf and 50 hpf. Arrows indicate CS cells that have undergone nuclear export of Hnf1b. Histogram on the right indicates ‘corrected total nuclear fluorescence’ (CTNF) of nuclei in the tubule versus nuclei in the CS gland at 22 hpf (n = 8). (B) Top panels show lateral views and bottom panels show dorsal views of wild-type and hnf1b-deficient embryos stained for stc1.
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