ZFIN Image: Naylor et al., 2018, Fig. 2

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Figure Caption

Fig. 2

CS extrusion is mediated by apical constriction.

(A) Panels show lateral views of the markers indicated in the region of the trunk where the CS will be derived (at 22 hpf) and is extruded (at 50 hpf). (B) Panels show lateral views of the trunk from live Tg(cdh17:egfp) embryos (left) and embryos stained for cdh17 (red)/stc1 (purple) transcripts (right) in control and Blebbistatin treated embryos. Black arrow indicates the position of stc1⁺ cells in the Blebbistatin treated embryo. (C) Panels show brightfield/fluorescence in Tg(cdh17:egfp) embryos before and after laser ablation (site of ablation indicated with asterisk). Panels on the right show embryos 18 hr post-ablation and arrowheads indicate the extruded CS gland.

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Acknowledgments

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