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Fig. 5
irx3b depletion causes aberrant pronephric morphogenesis.
(A) Lateral views of live un-injected and irx3b morphant Tg(cdh17::egfp) embryos with lower panels showing higher magnification of indicated pronephric regions. Histograms show the width of the tubule (left) and length of tubule cells (right) in the PST and DL regions of wild-type and irx3b morphants (n = 10). (B) Panels show dorsal view images of embryos stained for atp1a1a.4 at the stages indicated. Double-ended black arrows highlight areas of reduced atp1a1a.4 expression immediately anterior to a thickened distal tubule (yellow arrow) at 24 hpf and 50 hpf in irx3b morphants. (C) Lateral views of the trunk of Tg(cdh17:egfp) embryos (left panels) and embryos double stained for cdh17 (red) and stc1 (purple; right panels) after the indicated treatments. Black arrow highlights the ectopic stc1+ cells that remain in the tubule after irx3b knock down and Blebbistatin treatment.