ZFIN Image: Gordon et al., 2018, Fig. 7

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Figure Caption

Fig. 7

Canonical signaling via Gli1 is required for coloboma in the ptch2^tc294z mutant. (A) Inhibition of non-canonical Hh signaling via Src-family kinases (SFKs) does not rescue coloboma in the ptch2^tc294z mutant. Graph shows percentage of ptch2^tc294z mutant embryos with coloboma, normalized to the DMSO control group. (B) Genetic loss of one or both copies of gli1 (using gli1^ts269) partially rescues the coloboma phenotype in the ptch2^tc294z mutant. Percentage of embryos with coloboma is shown. Numbers in parentheses indicate n. (C-F) Representative images of embryos from the genetic experiments shown in B, 55 hpf. (C) ptch2^+/−; gli1^any. (D) ptch2^−/−; gli1^+/+. (E) ptch2^−/−; gli1^+/−. (F) ptch2^−/−; gli1^−/−. Arrow indicates coloboma.

Figure Data

Phenotype details

Acknowledgments

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