ZFIN Image: Ma et al., 2018, Fig. S4

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Figure Caption

Fig. S4

Sclerotome development in iguana (igu^-/-) mutants.

igu^-/- mutants and their sibling controls (wt or igu^+/-) were stained with nkx3.1, pax9, pax1a, and nkx3.2 at 24 hpf. In wt or igu^+/- controls, nkx3.1 and pax9 are expressed in the dorsal sclerotome domain (short arrows), the ventral sclerotome domain (long arrows), and sclerotome derived notochord associated cells (arrowheads), while pax1a and nkx3.2 are expressed in sclerotome derived notochord associated cells only. In igu^-/- mutants, expression of all four sclerotome markers are absent or significantly reduced in sclerotome derived notochord associated cells, while expression of nkx3.1 and pax9 in the dorsal and ventral sclerotome domains remains unchanged. Images shown are lateral views with close-up views of boxed regions. Brackets indicate the location of the notochord. n = 30 embryos per staining. Scale bars: 200 μm.

Acknowledgments

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