|
Fig. 7
Effect of nucleoplasmin (npm) 2a and npm2b deficiencies on zebrafish embryogenesis. Representative images demonstrating development of fertilized eggs from crosses between control (a-d), npm2a (e-l), or npm2b (m-p) females and wildtype (WT) males from 2 to 24 h post-fertilization (hpf). In the control eggs, the embryos were at 64-cell (a), oblong (b), germ ring (c), and 24-somite (d) stages according to Kimmel et al. [23]. Eggs from npm2a mutant females were non-developing with a normal morphology (e-h) or with an abnormal morphology (i-l). Eggs from npm2b mutant females had a normal morphology albeit were developing abnormally (e-h). (a, e, i, m) = images taken at 2 hpf; (b, f, j, n) = images taken at 4 hpf; (c, g, k, o) = images taken at 6 hpf; (d, h, l, p) = images taken at 24 hpf. Scale bars denote 400 μm. Red dotted lines define the diameter of the embryo. Arrows demonstrate a partially cellularized blastodisc that was sitting atop an enlarged yolk syncytial layer