ZFIN Image: Xu et al., 2018, Fig. 3

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Figure Caption

Fig. 3

TBK1/IKK? inhibitors promote ?-cell replication. (A?D) Confocal images of [Tg(ins:CFP-NTR)^s892; Tg(ins:Kaede)^jh6] larvae at 48 hpa, concurrently treated with EdU and DMSO (A), BX795 (B), amlexanox (C), or PIAA (D), respectively, from 0?48 hpa. The number of ?-cells that incorporated EdU (white arrows) was substantially increased in TBK1/IKK?-I-treated recovering larvae (B?D) compared to DMSO-treated larvae (A). (E) Quantification of the number (mean?�?SD) of total regenerated ?-cells (green) and regenerated ?-cells that incorporated EdU (yellow) at 48 hpa (in A-D; 5.0?�?1.3 total regenerated ?-cells, of which 0.7?�?0.5 (DMSO), 11.0?�?3.4, of which 4.6?�?1.8 (BX795), 12.8?�?4.8, of which 5.2?�?2.8 (amlexanox), and 20.6?�?3.1, of which 11.0?�?1.9 (PIAA) incorporated EdU). (F) The percentage (mean?�?SD) of regenerated ?-cells that incorporated EdU at 48 hpa (in A-D; 13.0?�?11.0% (DMSO), 42.0?�?5.0% (BX795), 39.0?�?7.0% (amlexanox), and 55.0?�?14.0% (PIAA)). Cells in 20 planes of confocal images from 10 individual larvae were counted per condition. **P?<?0.01; ***P?<?0.001.

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