ZFIN Image: Petratou et al., 2018, Fig. S2

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Figure Caption

Fig. S2

The mean cytoplasmic volume of cells in the dorsal ARPT can be calculated using high resolution DIC images combined with DAPI staining.

(A) DIC image of a single focal plane from a Z-stack, showing the ARPT of a 24 hpf WT embryo. (B) Magnified view of the boxed region in (A). DIC allows for identification of the boundaries of the cells directly dorsal to the CNS (likely epidermal), while DAPI stain renders the nuclei visible. It is thus possible to measure the length (L) and width (W) of whole cells (arrowheads), as well as of their respective nuclei. CNS, central nervous system; no, notochord; YSE, yolk sac extension. Lateral view, head positioned towards the left. Scale bar: A: 50 μm; B: 20 μm.

Acknowledgments

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