ZFIN Image: Ranski et al., 2018, Fig. 6

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Fig. 6

Following six rounds of light treatment, retinas replace the lost photoreceptors.(A?L) Retinal sections collected at 28 dpL immunolabeled with anti-rhodopsin, Zpr-1, anti-blue opsin, and anti-UV opsin to show regeneration of the photoreceptors in untreated control (No Light Treatment), experimental control (1�Light Treatment), and experimental retinas (1�Light Treatment) following photolytic damage. S Nuclei are stained blue with TO-PRO-3. (A?C). Rod photoreceptor outer segments are immunolabeled with anti-rhodopsin (red). (D?F) Red-green double cones are immunolabeled with Zpr-1 (green). (G?I) Long single cones are immunolabeled with anti-blue opsin (green). (J?L) Short single cones are immunolabeled with anti-UV opsin (green). (M?P) Quantification of the average number of photoreceptors 28 dpL in control and experimental groups (n = 5). Cells were counted over a linear distance of 300 �m on the central dorsal retina. Single asterisks indicate significant differences between groups (p < 0.03). Scale bar represents 25 ?m.

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Acknowledgments

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