ZFIN Image: Fernandez et al., 2018, Fig. S2

Image

Figure Caption

Fig. S2

bud13 knock-down show stronger cell death phenotype.

(A) Lateral view of WT embryos injected with 0.6mM of morpholino antisense oligonucleotide against bud13 mRNA (MObud13) showing different levels of developmental defects (types I to III). Phenotypes are fully rescue with human hBUD13 mRNA. (scale bar: 0.5mm). WT: represent phenotypically wild type sibling from the same mutant fish line. Stronger phenotype is likely due to a depletion of the maternal contribution. (B) bud13 mutant embryos fully rescued by providing 75 pg of hBUD13 mRNA, suggesting that Bud13 function may be conserved across vertebrates (scale bar: 0.5mm). WT: represent phenotypically wild type sibling from the same mutant fish line. (C) 48 hpf, bud13^Δ7/Δ7 embryos showing a similar phenotype to rbmx2^Δ16/Δ16 and snip1^Δ11/Δ11 when partly rescued by injection of 2.5 pg of hBUD13 mRNA (scale bar: 1mm). WT: represent phenotypically wild type sibling from the same mutant fish line.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.