ZFIN Image: Willoughby et al., 2012, Fig. 4

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Figure Caption

Fig. 4

Expression of HA-mCherry^TM in photoreceptors after heat-shock at 5 dpf.

Confocal z-projections of Tg(hsp70:HA-mCherry^TM) photoreceptor layers labeled with anti-HA antibody (red), anti-Rhodopsin antibody (blue) and with GFP-expressing rods (green). (A) At 6 dpf, 1 dpHS, HA-mCherry^TM in rod outer segments is seen as a stripe, an oval or a circle, depending on the orientation of the outer segment to the plane of section. (C) A magnification of the boxed area in (A) shows the displacement of the HA-mCherry^TM stripe from the base of the outer segment in 2-dimensions. (B) At 8 dpf, 3 dpHS, HA-mCherry^TM in rod outer segments is seen as a stripe, an oval or a circle, depending on the orientation of the outer segment to the plane of section and HA-mCherry^TM has moved distally compared to that seen at 1 dpHS. (D) A magnification of the boxed area in (B) shows the displacement of the HA-mCherry^TM stripe from the base of the outer segment in 2-dimensions. Scale bars: 5 µm, A, B; 5 µm, C, D.

Acknowledgments

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