ZFIN Image: Willoughby et al., 2012, Fig. 2

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Figure Caption

Fig. 2

Expression of HA-mCherry^TM in the eye of a 69.5 hpf larva at 1.5 hours hpHS (heat shocked at 68 hpf).

(A, C, E, G) Single confocal z-section images of an eye labeled with anti-HA antibodies. (B, D, F, H) Single confocal z-section image of an eye labeled with anti-HA antibodies merged with DIC-like image. (A, B) Heat-shocked Tg(hsp70:HA-mCherry^TM) larva shows ubiquitous expression of HA-mCherry^TM in retinal cells. (C, D) Heat-shocked wild-type eye shows only weak autofluorescence. (E, F) In the absence of heat-shock, an eye of a Tg(hsp70:HA-mCherry^TM) larva shows only weak expression of HA-mCherry^TM in a small number of amacrine cells (arrows). (G, H) An eye of a wild-type larva shows weak autofluorescence in the absence of heat-shock. Scale bar: A–H, 50 µm.

Acknowledgments

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