ZFIN Image: Swaminathan et al., 2018, Fig. 4

Image

Figure Caption

Fig. 4

Defects in GABAergic Networks in depdc5 Knockout Zebrafish Brains

(A, C, and D) Images of transverse sections of 6-dpf depdc5^+/+ and depdc5^−/− embryos immunostained with GAD65/67 (A), gephyrin (C), and Nlgn2 (D) antibodies and counterstained with DAPI. Section level is displayed in the schematic drawing. Regions indicated in red boxes have been magnified to show the branching defects; N = 3, n = 3 embryos/genotype. Scale bars, 10 μm.

(B) Imaris-reconstructed 3D images with Gad65/67-positive GABAergic boutons shown in green projecting toward the nucleus (DAPI) shown in blue. Scale bar, 2 μm.

(E) Images of longitudinal sections of 6-dpf depdc5^+/+ and depdc5^−/− embryos immunostained with parvalbumin 7 antibody. Scale bar, 25 μm. Field of view is displayed in the schematic drawing.

(F) Quantification of the number of GAD65/67-positive neurofilaments, gephyrin-positive puncta, and parvalbumin-positive cells in depdc5^+/+ and ^−/− larval brain sections; N = 3, n > 3 embryos/genotype.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Curr. Biol.