ZFIN Image: Du et al., 2018, Fig. S2

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Figure Caption

Fig. S2

Confirmation of LC neuron death induced by two-photon laser-based ablation, Related to Figure 2.

(A) TUNEL signals in the ablated LC area in an ETvmat2:GFP larva. TUNEL staining was performed 1 hour after two-photon ablation. Top, fluorescent images showing GFP or TUNEL signal; bottom, bright field images showing bulb-like structures (white arrows). All images were obtained from the same larva. Scale: 50 μm.

(B) No GFP-positive cells in the ablated LC area in an ETvmat2:GFP larva 24 hours after two-photon ablation. Top, fluorescent images showing GFP signal; bottom, bright field images showing bulb-like structures (white arrows). All images were obtained from the same larva. Scale: 50 μm.

Acknowledgments

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