ZFIN Image: Zhang et al., 2018, Fig. S1

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Figure Caption

Fig. S1

Related to Figure 1 and 2: bace2^-/- mutants have increased mRNA expression of pigmentation genes.

(A) Schematic representation of zebrafish WT and Bace2-/- protein as found in the wanderlust mutant. Zebrafish Bace2 is a type I integral membrane β secretase that contains two DTG and DSG protease sites within its extracellular/lumenal domain. The wanderlust mutant (Bace2-/-) has a C to A nonsense mutation leading to a truncated protein with size of 56 amino acids (aa), lacking both protease sites. TM=Transmembrane domain. (B-C) ISH shows no detectable difference in neural crest or melanophore genes expression between WT and bace2-/- fish at 24 hpf (B), but ISH shows pigmentation genes are elevated in bace2-/- fish at 72 hpf (C). Scale bars, 200μM.

Acknowledgments

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Reprinted from Developmental Cell, 45(5), Zhang, Y.M., Zimmer, M.A., Guardia, T., Callahan, S.J., Mondal, C., Di Martino, J., Takagi, T., Fennell, M., Garippa, R., Campbell, N.R., Bravo-Cordero, J.J., White, R.M., Distant Insulin Signaling Regulates Vertebrate Pigmentation through the Sheddase Bace2, 580-594.e7, Copyright (2018) with permission from Elsevier. Full text @ Dev. Cell