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Fig. S7
Related to Figure 7: Pancreatic insa ablation does not rescue the bace2-/- phenotype.
(A-B) ISH shows insa mRNA is specifically expressed in pancreas at 24hpf (A) and at 72hpf (B) for both WT and bace2-/- embryos (arrowhead). (C) Immunostaining shows knockdown of either insa or pdx1/hb9 in bace2-/- mutants completely ablates insulin staining in the pancreas, while insb knockdown does not change pancreatic insulin level at 72hpf. Whole embryos were stained with anti-insulin antibody. (D) Co-knockdown of pdx1 and hb9 in bace2-/- mutants fails to rescue dendritic melanophores at 72hpf. (E) wanderlust mutant have slightly increased insa positive cell area compared to WT embryos at 72hpf, but Bace2 inhibitor and Bace2 morphant do not show significant differences to the control group, suggesting Bace2 loss of function does not consistently alter pancreatic β cell mass at 72hpf. n=each fish, two-tailed t test, *P<0.05. All bar graphs are presented as mean ± s.e.m. Scale bars, 50μM (C), 100μM (A, B, D).
Reprinted from Developmental Cell, 45(5), Zhang, Y.M., Zimmer, M.A., Guardia, T., Callahan, S.J., Mondal, C., Di Martino, J., Takagi, T., Fennell, M., Garippa, R., Campbell, N.R., Bravo-Cordero, J.J., White, R.M., Distant Insulin Signaling Regulates Vertebrate Pigmentation through the Sheddase Bace2, 580-594.e7, Copyright (2018) with permission from Elsevier. Full text @ Dev. Cell