ZFIN Image: Powell et al., 2018, Fig. 5

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Figure Caption

Fig. 5

Blocking neutrophil chemotaxis signaling reduces proliferation of tumor-initiating cells. cxcr1 heterozygote fish were in-crossed, injected with gfap:kras^G12V, fixed for pH3 immunostaining at 3 dpf, and genotyped post-imaging as described above (A). pH3-GFP double-positive cells were quantified and normalized as in previous figures (B, yellow arrowheads in A, n = 19 WT/het and 26 cxcr1−/− larvae). Wildtype larvae expressing gfap:kras^G12V were treated with 1 µM SB225002 or DMSO control as described in Fig. 4. Larvae were fixed at 3 dpf and immunostained for phospho-Histone H3 (C). Proliferating cells were quantified as above (D, double-positive cells marked by yellow arrowhead in C, n = 17 larvae/condition). *p < 0.05.

Acknowledgments

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