ZFIN Image: Powell et al., 2018, Fig. 3

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Figure Caption

Fig. 3

Blocking neutrophil motility reduces proliferation of tumor-initiating cells. Wildtype or Tg(mpx:mCherry) embryos were co-injected with gfap:GFP-kras^G12V plasmid and rac2 or standard control morpholino (MO). Larvae were live-imaged for neutrophil recruitment at 3 dpf (A) and quantified for neutrophils within the field of view normalized to GFP-expression area (B, n = 13 control MO and 18 rac2 MO larvae). Larvae were also fixed at 3 dpf and immunostained for phospho-Histone H3 (C). Double-positive (pH3 and GFP) cells were quantified by optical sectioning and normalized as above (D, yellow arrowheads in C, n = 21 control MO and 17 rac2 MO larvae). Tg(mpx:mCherry-2a-Rac2^WT) or Tg(mpx:mCherry-2a-Rac2^D57N) embryos were injected with gfap:GFP-kras^G12V and live-imaged at 3 dpf (E). mCherry-positive neutrophils were quantified and normalized as above (F, n = 52 Rac2^WT and 46 Rac2^D57N larvae). Larvae were fixed at 3 dpf and immunostained for phospho-Histone H3 (G). pH3-GFP double positive cells were quantified by optical section and normalized as above (H, n = 30 Rac2^WT and 37 Rac2^D57N larvae). *p < 0.05.

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Acknowledgments

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