ZFIN Image: Powell et al., 2018, Fig. 2

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Fig. 2

Neutrophils are recruited to the tumor-initiating microenvironment. Live-imaging of neutrophils in the hindbrain of Tg(mpx:mCherry) larvae expressing gfap:kras^WT or kras^G12V at 3 dpf (A). Neutrophils within the field of view were quantified and normalized to the total gfap-expressing area within the same field of view at 3 and 6 dpf (B, n = 24 kras^WT 3dpf, 42 kras^G12V 3 dpf, 24 kras^WT 6 dpf, and 30 kras^G12V 6 dpf larvae). Live time-lapse imaging was performed for 9 hours beginning at 3–3.5 dpf and total neutrophils recruited to the hindbrain during the period of imaging were quantified (C, n = 9 kras^WT and 11 kras^G12V larvae). qRT-PCR was performed on whole-larvae mRNA isolates for several neutrophil recruiting chemokines implicated in the tumor microenvironment including cxcl8a, cxcl8b, and il1b (D, n = 6 replicates for cxcl8a/b, 5 replicates for il1b, normalized as in Fig. 1D). *p < 0.05.

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Acknowledgments

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