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Fig. 2
Expression of ppp1r12a splice variants during early embryonic development in zebrafish. (A) Transcript specific primers were used to detect ppp1r12a-tv202 or to detect the long transcript variants tv-201 and tv-203, labelled tv-long. Embryos were collected immediately after fertilization (0 hpf), 64-cell stage (2 hpf), sphere stage (4 hpf), shield stage (6 hpf), bud stage (10 hpf), 6 somite stage (12 hpf), 10 somite stage (14 hpf), 22 somite stage (20 hpf), Prim-5 stage (24 hpf), long-pec stage (48 hpf) and protruding-month stage (72 hpf). Amplification of eF1a and total RNA without addition of reverse transcriptase were used as controls. The PCR products amplified by tv-long, tv202 and eF1a primers were 769 bp, 206 bp and 720 bp in length respectively. (B-D) The spatial expression of ppp1r12a-tv202 was analyzed by whole-mount in situ hybridization using a probe targeted at the unique regions of exon 12 and 13 in tv202 at the 64 cell stage (B), bud stage (C) and 24 hpf (D). Quantitative analysis of mRNA expression levels of long-tv (E) and tv202 (F) during zebrafish development. Values are means of three biological replicates performed in duplicate and error bars are standard deviation. All values are normalized the eF1a and reported as % of expression of the long-tv expression at 0 hpf.
Reprinted from Gene, 675, LaFlamme, A., Young, K.E., Lang, I., Weiser, D.C., Alternative splicing of (ppp1r12a/mypt1) in zebrafish produces a novel myosin phosphatase targeting subunit, 15-26, Copyright (2018) with permission from Elsevier. Full text @ Gene