ZFIN Image: Moore et al., 2013, Fig. S1

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Figure Caption

Fig. S1

Several functional CRMs are located within the 4^th intron of the Pax3 locus. (A) The full Mulan alignment of the Pax3 locus, summarised in Figure 1E. (B) CNE1 transient transgenics exhibit reporter expression across the AP axis of the developing CNS at 24 hpf (n = 67). (C) By contrast, CNE2 activity weakly labels postmitotic neurons within the dorsal spinal cord (n = 11). (D) At 24 hpf, CNE3 is active within the midbrain (mb), hindbrain (mb) and progenitors within the dorsal spinal cord (dP) (n = 51). (E) CNE4 is sufficient to direct transcription within muscle fibres (mf) and cranial neural crest (nc) (n = 32). (F) Surprisingly, CNE5 robustly labels the notochord (nc) and floor plate (fp) of the neural tube, tissues that do not express Pax3 at any point of their development (n = 34).

Acknowledgments

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