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Fig. 6
Effect of vegfaa overexpression on heart regeneration. (A–E) Tilescan images of cmlc2:CreER;βact2:BS-vegfaa hearts at 7 dpa after treatment with vehicle or tamoxifen. Boxes mark the regions at the injury site (B and E) and away from the injury site (C and F). Arrowheads indicate Mef2+/PCNA+ nuclei. PCNA+ nuclei that do not colocalize with Mef2 are likely to be cycling epicardial cells, endothelial cells, fibroblasts, or inflammatory cells. (G and H) CM proliferation adjacent to the wound (G) and throughout the ventricle (H) at 7 dpa and after treatment with vehicle (n = 8) or tamoxifen (n = 12). Error bars indicate SEM (*P < 0.05 and **P < 0.01, Mann–Whitney U test). (I–N) cmlc2:CreER;βact2:BS-vegfaa;fli1a:EGFP ventricles at 30 dpa. Tnnt marks CMs. Boxes mark the regions at the injury site (K and N) and away from the injury (I and L). Dashed lines in K and N approximate the resection plane. Dashed lines in I and L indicate the boundary of trabecular muscle and compact muscle. (O) Regeneration scores at 30 dpa after treatment with vehicle (n = 14) or tamoxifen (n = 17; P < 0.001, Kruskal–Wallis test). [Scale bars: A and J, 100 μm (also apply to D and M, respectively); B, C, I, and K, 25 μm (also apply to E, F, L, and N, respectively).]