ZFIN Image: Karra et al., 2018, Fig. 4

Image

Figure Caption

Fig. 4

Cardiac vegfaa overexpression elicits an ectopic heart regeneration program. (A and B) Tiled images of cmlc2:CreER;?act2:BS-vegfaa hearts 14 dpi with vehicle or tamoxifen. Arrowheads indicate Mef2⁺/PCNA⁺ nuclei. Mef2^?/PCNA⁺ nuclei are likely to be cycling epicardial cells, endothelial cells, fibroblasts, or inflammatory cells. (C) CM proliferation 14 dpi with vehicle (n = 10) or tamoxifen (n = 8). Error bars indicate SEM (***P < 0.001, Mann?Whitney U test). (D and E) cmlc2:CreER;?act2:BS-vegfaa;gata4:EGFP hearts 14 dpi with vehicle or tamoxifen. Arrowheads indicate gata4⁺ CMs. (F and G) Images of cmlc2:CreER;?act2:BS-vegfaa;LENP2:EGFP hearts 14 dpi with vehicle or tamoxifen. Arrowheads indicate LENP2⁺ cells. Tnnt marks CMs. Boxes correspond to the region magnified in adjacent panels. (H and I) Images of cmlc2:CreER;?act2:BS-vegfaa;tcf21:DsRed hearts 14 dpi with vehicle or tamoxifen. (J and K) In situ hybridization for raldh2 on ventricular sections. Violet staining indicates expression. [Scale bars: B, H, and J, 100 ?m (also apply to A, I, and K, respectively); D and F, 100 ?m (Left) and 25 ?m (Right) (also apply to E and G, respectively).]

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA