ZFIN Image: Karra et al., 2018, Fig. 3

Image

Figure Caption

Fig. 3

Effects of cardiac vegfaa overexpression in the absence of injury. (A–D) Tiled images of cmlc2:CreER;βact2:BS-vegfaa;fli1a:EGFP hearts at 6 wpf and 8 wpf after treatment with vehicle or tamoxifen at 30 dpf. (E and F) Tiled images of adult cmlc2:CreER;βact2:BS-vegfaa;fli1a:EGFP hearts 30 dpi with vehicle or tamoxifen. Sections are immunostained for Tnnt. Boxes correspond to the region magnified in adjacent panels. Blue lines approximate the CM primordial layer and blue arrows denote ectopic vasculature. (G–I) Maximum thickness of the Tnnt⁺ compact layer at 6 wpf after treating 30-dpf fish with vehicle (n = 11) or tamoxifen (n = 6); at 8 wpf after treating 30-dpf fish with vehicle (n = 11) or tamoxifen (n = 9); and at 30 dpi after treating adult fish with vehicle (n = 5) or tamoxifen (n = 6). Error bars indicate SD (**P < 0.01 and ***P < 0.001, Student’s t test). (Scale bars: A–F, Left, 100 μm; A–F, Right, 25 μm.)

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA