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Fig. S5
Hemogen mutant zebrafish have increased cell death during embryonic development.
(A) Genotypic ratios of 2 dpf embryos produced from heterozygous incrosses of Hemgnnuz3 (?5) or Hemgnnuz4 (?12) mutants. (B) Proportion of genotyped mutants and wild-type sibling embryos at 2 dpf that were apoptotic (black) or phenotypically normal (white) (* P ? 0.05, ** P ? 0.005, Chi square). (BC) Acridine orange staining for apoptotic cells is increased in the bodies and in the peripheral blood island (outlined) in 20 hpf heterozygous Hemgnnuz2 mutant zebrafish (n = 3) compared to wild-type siblings (n = 3). (D) Comparison of adult wild-type and homozygous Hemgnnuz4 (?12) mutant. (E) Average body length of adult wild-type and Hemgnnuz4 (?12) mutants. Error bars represent standard error (*, P ? 0.05, Student?s t test). Scale bars = 100 ?m (E), 50 mm (D)