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Fig. S6
Effects of suppressing Fn or/and Lam expression on endoderm C&E.
(A) Expression of lam1 (a1, b1a, b1b, c1) and fn (1a and 1b) relative to that of the housekeeping gene eukaryotic translation elongation factor 1 alpha 1a (eef1a) in WT embryos at TB and 4s, as determined by qRT-PCR. (B-B) Confocal z-stack images of transverse cryosections immunostained for Lam (magenta) and nuclei (DAPI, blue) from the indicated embryos. (C) Western blot showing expression levels of Fn and Hspa9 (internal control) in embryos indicated. (D-I) Epifluorescence still images of the anterior endodermal sheet in embryos indicated. Anterior-dorsal view. A, anterior; P, posterior. White lines of equivalent length indicate width of anterior endodermal sheet of the embryos at the same stage. (J,K) Average width of anterior endoderm. (J) Embryos injected with the indicated MO (10 ng), shown in (D-F). (K) Embryos treated as indicated and shown in (G-I). Number of embryos analyzed is indicated for each group. #, p>0.05, **, p<0.01, ****, p<0.0001, student’s t-test.