ZFIN Image: Rosowski et al., 2018, Fig. 3

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Figure Caption

Fig. 3

CEA10-induced inflammatory activation is not sufficient for killing of an Af293-derived strain.

A. Fungal burden was monitored by CFU platings from single homogenized larvae infected either singly with TFYL49.1 (CEA10) or TBK5.1 (Af293) or co-infected with both strains. Average injection CFUs: Af293 = 53, CEA10 = 47, co-infection = 21 Af293 + 27 CEA10. CFUs from 24 larvae (8 larvae per replicate) per condition per day were measured; lsmeans ± SEM are shown from three pooled experiments, P values calculated by ANOVA. B, C. Macrophage-membrane labeled larvae (mfap4:tomato-CAAX) were co-infected with YFP-expressing TBK1.1 (Af293) and AlexaFluor633-labeled CEA17 KU80Δ (CEA10) and imaged 1 dpi. Percentage of Af293 spores within 20 μm of a CEA10 spore in each larvae from two replicates was quantified (B). Each point represents one larvae, color-coded by replicate. Single z-slice images from a representative larvae (C) are shown. Scale bar represents 5 μm. See also S3 Fig.

Acknowledgments

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