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Fig. 1
A CEA10-derived strain is killed more than an Af293-derived strain in vivo.
A. Schematic of 2 dpf zebrafish larvae. Area of Aspergillus spore injection (hindbrain) and imaging is indicated. B, C. TBK1.1 (Af293) or TFYL49.1 (CEA10) spores express YFP or GFP and have AlexaFluor594 conjugated to the spore cell wall. 2 dpi larvae were imaged and the percentage of spores killed (GFP+/total) was quantified. Representative images of single z slices (B) and quantification over three experiments (C) are shown. Arrowheads indicate alive spores, asterisks indicate killed spores. Scale bar represents 10 μm. Each symbol represents one larvae, color-coded by experiment. Lines represent lsmeans ± SEM. Af293 n = 51, CEA10 n = 45. D. Fungal burden was monitored by CFU platings from single homogenized larvae infected with TBK1.1 (Af293) or TFYL49.1 (CEA10). CFUs from 24 larvae (3 replicates, 8 larvae each) per condition per day were measured. Average injection CFUs: Af293 = 150, CEA10 = 140. For all analyses, data represent lsmeans ± SEM from three pooled experiments, P values calculated by ANOVA. Asterisks represent significance between strains, # represent significance compared to day 0. See also S1 Fig.