ZFIN Image: Nichols et al., 2018, Fig. 6

Image

Figure Caption

Fig. 6

Extended perturbations of soma-ensheathing cells results in misplaced neurons. (a). Confocal z-projection images from a 24-h timelapse starting at 48 hpf of a Tg(foxd3:mcherry) zebrafish. White arrows denote the center of the neuronal soma. (b). Confocal z-projection images from a 24-h timelapse starting at 72 hpf of a Tg(sox10:eos) zebrafish with a laser ablation of multiple ensheathing cells. White arrows denote the original location of the dorsal apex of the DRG neuron. (c). Change in the y-position over time of a neuron in an ablated and unablated DRG. Y = 0 denotes the final location of the neuronal soma. (d). Total distance traveled by the DRG neuronal soma in an ablated and unablated DRG (n = 5 neurons). (e). Confocal z-projection images from a 24-h timelapse starting at 48 hpf of a Tg(sox10:eos) zebrafish with a photoconverted neuron and ablated ensheathing cell. White arrows denote the migration of the nascent axon. (d) use an unpaired Student’s t-test. Scale bar is 10 μm (a, b, e)

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Neural Dev.