ZFIN Image: Gurung et al., 2018, Fig. S3

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Figure Caption

Fig. S3

Outgrowth of Rohon-Beard central axons is not affected in MZcntn2 mutants

(A) Embryos injected with ngn1:GFP-caax DNA and Tol2 transposase RNA were fixed at 18 hpf, and processed for immunohistochemistry with anti-GFP antibody to label Rohon-Beard (RB) neurons . Asterisk indicates cell body, and the filled and open arrowheads indicate ascending and descending central axons, respectively, of a labeled RB neuron. Scale bar, 50 μm. (B-D) Quantification of the lengths of ascending and descending central axons, and total length (sum of ascending and descending lengths) of central axons of RB neurons located in the anterior (somites 1-5), intermediate (somite 6-10) and posterior (somites 11-16) regions of the spinal cord. There were no significant differences in the length of RB central axons between wildtype and MZcntn2 mutant embryos. Number of RB neurons scored is shown in parenthesis. Unpaired t-test; NS: not significant. Error bars show Mean ± SEM.

Acknowledgments

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Reprinted from Mechanisms of Development, 152, Gurung, S., Asante, E., Hummel, D., Williams, A., Feldman-Schultz, O., Halloran, M.C., Sittaramane, V., Chandrasekhar, A., Distinct roles for the cell adhesion molecule Contactin2 in the development and function of neural circuits in zebrafish, 1-12, Copyright (2018) with permission from Elsevier. Full text @ Mech. Dev.