ZFIN Image: Genthe et al., 2017, Fig. 3

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Figure Caption

Fig. 3

Notch pathway gene expression in Rspo1 morphants. Wild-type (A,D,G,J,M,P) or Rspo1 MO-injected animals (B,E,H,K,N,Q) were examined by WISH for expression of the Notch ligands dlc (A,B), dld (D,E), or Notch receptors notch1a (G,H), notch1b (J,K), notch2 (M,N) and notch3 (P,Q) at the time points indicated. Compared with wild-type (A,D), dlc and dld are specifically decreased (B,E) in the formed somites (below red bars), but not the pre-somitic mesoderm. Notch receptor expression is unaffected. Flat mount dorsal views of the trunk or lateral views at 160× magnification. Numbers of embryos displaying the depicted phenotype are indicated. (C,F,I,L,O,R) Histograms illustrate percentages of phenotypes. (S,T) Decreased dlc and dld mRNA in Rspo1 knockdown (Rspo1 MO) was confirmed by qRT-PCR, normalized to expression of ef1a, of transcript isolated from dissected embryo trunks (mean±s.e.m.). *P≤0.0006, determined by one-way ANOVA.

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Acknowledgments

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