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Fig. S6
Vegfa does not rescue dlc and dld expression in Rspo1 morphants. (A) Lateral view image of wild-type 17 hpf embryo with box indicating the location of in situ images. The cartoon shows a flat mount section of the tail of the embryo with formed somites and the pre-somitic mesoderm indicated. Wild-type (B,F), Rspo1 MO (C,G), Rspo1 MO + vegfaa-165 mRNA (D,H), or Rspo1 MO + vegfaa-121 mRNA (E,I) were examined by WISH for expression dlc (B-E) or dld (F-I). Numbers of embryos displaying the depicted phenotype are indicated. Magnification 160X. (J-K) dlc and dld mRNA (mean±SEM) levels relative to ef1 control quantified by qRT-PCR from uninjected (WT), Rspo1 MO Rspo1 MO + vegfaa-165 mRNA and Rspo1 MO + vegfaa-121 mRNA injected animals. * significantly different from WT (p ≤ 0.005) determined by one-way ANOVA.