Fig. 3

Fig. 3

Protective effects of tanshinol on head bone mineralisation of wild-type AB strains zebrafish larval exposed to dexamethasone (Dex). Images of (A) the dorsal aspect head bone stained with alizarin red in wild-type AB strains zebrafish larvae at 9 days' postfertilisation (dpf) exposed to Dex (10μM) in the presence or absence of tanshinol; (B) the effect of tanshinol on bone mineralisation area in 9-dpf zebrafish exposed to Dex; and (C) the effect of tanshinol on bone mineralisation integral optical density in 9-dpf zebrafish exposed to Dex. Dex = Dex 10μM; D+R = Dex + rocalirol 6 × 10⁻⁶ μg/mL); D+T₁ = Dex + tanshinol 1μM; D+T_2.5 = Dex + tanshinol 2.5μM; D+T₅ = Dex + tanshinol 5μM; D+T₁₀ = Dex + tanshinol 10μM; D+T₅₀ = Dex + tanshinol 50μM; IOD = integral optical density; Veh = vehicle control, 0.1% dimethyl sulphoxide. Data are given as mean ± standard deviation (n ≥ 9). *p < 0.05 versus vehicle control. **p < 0.05 versus Dex treatment.