ZFIN Image: Luo et al., 2015, Fig. 2

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Fig. 2

Effects of tanshinol on bone mineralisation of wild-type AB strains zebrafish larval skull. Images of (A) the dorsal aspect head bone stained with alizarin red in wild-type AB strains zebrafish larvae at 9 days' postfertilisation (dpf) with or without exposure to tanshinol (0.5μM, 1μM, 2.5μM, 5μM); (B) the effect of tanshinol on bone mineralisation area in 9-dpf zebrafish; and (C) the effect of tanshinol on bone mineralisation integral optical density in 9-dpf zebrafish. Con = control, egg water; IOD = integral optical density; T_0.5 = tanshinol 0.5μM; T₁ = tanshinol 1μM; T_2.5 = tanshinol 2.5μM; T₅ = tanshinol 5μM. Data are given as mean ± standard deviation (n ≥ 9). *p < 0.05 versus control.

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