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Fig. 2
The effects of triptolide on podocin expression and distribution in MTZ-treated Tg(pod:gal4;UAS:NTR-mCherry) zebrafish. (A) The expression and distribution of podocin in different groups was measured and examined at 24 h, 30 h and 36 h after MTZ treatment in 84 hpf larvae; (a1–a3) normal control (CTL); (b1–b3) MTZ model group; (c1–c3) MTZ + triptolide group. (B) No obvious mean immunofluorescence intensity (GL) differences were observed between the three groups at 30 h and 36 h. (C) Positively stained area to the glomerular area (AR) revealed a remarkably reduction at 36 h in the MTZ model group, but remained strong in the triptolide treatment group. (n = 3), **P < 0.01, (original magnification, x1000).