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Fig. 3
Compound 8 ameliorates the CE and jaw defects caused by loss of rap1 and kdm6a. (a) gRNA targeting both zebrafish rap1a and rap1b were injected into embryos at the 1-cell stage with Cas9 protein. Larvae were incubated with compound 8 (100 nM) at the 8-cell stage and scored for micrognathia. 5 dpf larvae were stained with Alcian blue to assess the layout of the jaw cartilage. In comparison to control embryos (top), rap1-CRISPR embryos have smaller jaws. This phenotype can be ameliorated by treatment with compound 2 and 8. (b) Quantitative measurement of the distance between MK and CH cartilages. (c) Larvae were scored for CE (n > 30 for each concentration). (d) 5 dpf embryos were stained with Alcian blue to assess the layout of jaw cartilage. In comparison to control embryos (top), kdm6a-MO embryos exhibit smaller jaws. This phenotype can be ameliorated by treatment with compound 8. (e) Quantitative measurement of the distance between MK and CH cartilages. *p < 0.05; ***p < 0.001. Error bars show SEM (standard error of the mean).