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Fig. 1
Establishment of a zebrafish larva GIOP model using 25 μM prednisolone. (A-F) 8−10 dpf zebrafish treated with 25 μM prednisolone. Whole mount skeletal staining was performed on fixed tissues. The mineralized tissue is stained purple and all other tissues are transparent. (G-I) Digital image analysis of stained area and staining density by assessment of fluorescence intensity. The stained mineralized tissue was quantified using analysis software. Mean values are plotted (n=10) and the Student's t-test was performed to determine statistical significance.
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