ZFIN Image: Gauvrit et al., 2018, Fig. 1

Image

Figure Caption

Fig. 1

Zebrafish hhex mutants lack sprouting angiogenesis from the posterior cardinal vein. a Schematic representation of Hhex. Hhex, 228 amino acids (aa) long, is composed of a proline-rich domain (4–113 aa) and a homeodomain (116–175 aa). b Alignment of partial Hhex homeodomain sequence in wild-type (WT), and two mutant alleles, hhex^s721 and hhex^s722. The hhex^s721 allele contains a 10 bp insertion leading to a premature stop codon within the homeodomain coding region, whereas the hhex^s722 allele lacks amino acids R149 to A151. c, d Trunk vasculature of Tg(−5.2lyve1b:DsRed); hhex^+/+ and hhex^−/− embryos at 48 hpf. hhex mutant trunks exhibit a defect in sprouting angiogenesis from the posterior cardinal vein (PCV) (arrowheads point to tip cells sprouting from the PCV; asterisks indicate lack of tip cells sprouting from the PCV). e, f Trunk vasculature of Tg(−5.2lyve1b:DsRed); hhex^+/+ and hhex^−/− larvae at 5 dpf. hhex mutant trunks exhibit a defect in the formation of the venous intersegmental vessels (vISVs), the thoracic duct (TD) lymphatic vessel, and the dorsal longitudinal lymphatic vessel (DLLV) (arrowhead points to a vISV; arrows point to the ventrally positioned TD and dorsally positioned DLLV; asterisks indicate lack of these structures). g, h Brightfield lateral views of hhex^+/+ and hhex^−/− larvae at 5 dpf. Mutant larvae exhibit pericardial edema (arrowhead). Scale bars: 100 μm

Figure Data

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.