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Fig. S2
Analysis of egr2 knockdown embryos
(A-H): Knockdown of egr2a and egr2b results in loss of egr2b expression in r3 and severe reduction of expression in r5* at 17 hpf (E) compared to control embryos (A). Following loss of r3 territory, the flanking segments still express hox genes that mark r2 (hoxa2) and r4 (hoxb1 plus hoxa2): compare controls (B-D) with egr2 knockdown embryos (F-H). A-C and E-G are single in situ hybridizations, and D, H are double in situ hybridizations, as indicated. Scale bar: 50 μm.
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Reprinted from Developmental Cell, 45, Addison, M., Xu, Q., Cayuso, J., Wilkinson, D.G., Cell Identity Switching Regulated by Retinoic Acid Signaling Maintains Homogeneous Segments in the Hindbrain, 606-620.e3, Copyright (2018) with permission from Elsevier. Full text @ Dev. Cell