ZFIN Image: Astone et al., 2018, Fig. 1

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Figure Caption

Fig. 1

Hsa.CTGF-based Yap1/Taz zebrafish reporter line. (A) ctgf promoter contains three Yap1/Taz responsive elements (RE) and is remarkably conserved between zebrafish and humans. The promoter region upstream of the 5′UTR (uppercase) of the zebrafish ctgfa and human CTGF genes contain each three TEAD DNA-binding sites (YAP1/TAZ REs; in light blue) just before the TATA box (in yellow). Their sequences, orientation and distances are remarkably conserved between zebrafish and humans. (B) Schematic representation of the Hsa.CTGF-based Yap1/Taz reporter construct. The Yap1/Taz-responsive fragment of the construct is derived from the human CTGF promoter. It contains the pathway-specific REs MCAT and the TATA box, and regulates the expression of the downstream reporter gene. (C) Overview of a Tg(Hsa.CTGF:nlsmCherry) larva.

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