ZFIN Image: Ferrero et al., 2018, Fig. 1

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Figure Caption

Fig. 1

MG Identity Is Conserved in the Zebrafish

(A) Fluorescence of GFP (left panel) and DsRed (middle panel) in the brain of adult cd45:DsRed; mhc2dab:GFP double-transgenic fish. The right panel shows a merge of both fluorescent channels (n = 3). Scale bar: 10 μm.

(B) Flow cytometry analysis on brain cell suspensions from adult Tg(cd45:DsRed; mhc2dab:GFP) identifying cd45:DsRed^lowmhc2dab:GFP⁺ MG (red gate, MG) and cd45:DsRed^highmhc2dab:GFP⁺ CNS-associated macrophages (blue gate, MFs).

(C) Comparison of the relative expression of apoeb, p2ry12, csf1ra, C1qc, csf3r, mpeg1, pu.1/spi1b, and cd45/ptprc transcripts between MG (red bars) and MFs (blue bars) cell subsets isolated by FACS. Error bars represent mean ± SD (n = 3).

(D and E) Flow cytometry of a brain cell suspension from adult mpeg1:GFP transgenics (D) and expression of apoeb, p2ry12, csf1ra, and mpeg1 by qPCR in FACS-sorted mpeg1:GFP⁺ cell subsets (E). Error bars represent mean ± SD (n = 3).

(F) GFP immunohistochemistry on adult mpeg1:GFP brain sections. Scale bar: 25 μm.

(G) apoeb expression in mpeg1:GFP⁺ cells sorted at 1, 2, and 3 dpf. MG, microglia; MFs, macrophages.

Figure Data

Expression / Labeling details

Acknowledgments

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