ZFIN Image: Collins et al., 2018, Fig. 2-S1

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Figure Caption

Fig. 2-S1

Proliferation is reduced in MZpitx2c mutant embryos during gastrulation.

(a, b) Immunostaining of wild-type (a) and MZpitx2c mutant (b) embryos for phospho-Histone H3 (pH3) and counterstaining with DAPI to visualize nuclei. (c) Quantification of the number of pH3⁺ cells in wild-type and MZpitx2c mutant embryos at 80–90% epiboly. (d, e) Immunostaining of wild-type (d) and MZpitx2c mutant (e) embryos for cleaved Caspase-3 and counterstaining with DAPI to visualize nuclei. (f) Quantification of the number of Caspase-3⁺ cells in wild-type and MZpitx2c mutant embryos at 80–90% epiboly. Scale bars, 100 μm. *p<0.05 by unpaired t-test.

Acknowledgments

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