ZFIN Image: Guzzolino et al., 2018, Fig. 1

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Figure Caption

Fig. 1

s1pr1 overexpression affects vascular development. (A) Confocal microscopy of representative 48 hpf Tg(kdrl:EGFP)^s843 embryos showing vascular defects of different severity. The Tg(kdrl:EGFP)^s843 embryos were injected with in vitro transcribed and capped mRNA of the full-length (CDS+UTR) or the CDS sequence of s1pr1 at the reported doses (pg). The total numbers of embryos analyzed were as follows: 300 pg RFP CDS injected n = 20; 150 pg s1pr1 CDS injected n = 22; 300 pg s1pr1 CDS injected n = 38; 300 pg s1pr1 CDS+UTR injected n = 15. Red arrows indicate examples of shorter and incomplete ISVs. Scale bar = 500 μm (zoom 100 μm). (B) Blinded quantification of the abnormal vascular phenotypes showed in (A). **P < 0.001, ***P < 0.0001.

Acknowledgments

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