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Fig. 1 (A) Relative expression of zfMGST1a/b during zebrafish embryonic development. (B) Relative activity of GST enzymes during zebrafish embryonic development. (C) Expression of MGST1a and MGST1b in the intermediate cell mass of 24 hpf embryos. (D) MGST1a/b protein colocalizes with cmyb, a marker specific for HSCs, in 48 hpf embryos. Confocal images were taken on a Leica LSM700 with a 20× lens; Alexa 488 and 555 filters were used; images stacks were produced with ImageJ, GIMP was used to adjust the gamma channel.
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