ZFIN Image: Mahony et al., 2018, Fig. S4

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Figure Caption

Fig. S4

Characterisation of ikaros^high and ikaros^low population. (A) Thymus imaging in ikaros:eGFP 4.5dpf embryos. P=0.0031. Scale, 50μm. NI, non-injected control. +osm, osm full length mRNA injected embryos. (B) FACS sorting of ikaros:eGFP^low and ikaros:eGFP^high cells at 48hpf from non-injected embryos. (C-P) qPCR analysis of gene expression in the low and high population. Data represented is mean±SD from biological triplicates, except in runx1, gata1 and osmr ikaros:eGFP^high where data is biological duplicates. Statistical analysis was completed using an unpaired, two-tailed Student's t-test. ** p<0.01.

Acknowledgments

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