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Fig. 5-S1
Overexpression of Cxcr4a in wild-type PGCs leads to increase in number of ectopic PGCs.
(A) Wild-type embryos expressing Cxcr4b (left) or Cxcr4a (right) in their PGCs. (B) Graph showing percentage of ectopic PGCs per embryo. 80 pg of RNA encoding for either Cxcr4a or Cxcr4b was injected into the embryo. The inclusion of the nanos 3’UTR leads to preferential expression of the proteins in the PGCs. 60 pg of mGFP-nanos RNA was used to label PGCs in green. For raw data see Source data file Figure 5—figure supplement 1.
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